The Cytology of Amoeba proteus ' Y ' and the effects of large and small Centrifugal Force s

نویسندگان

  • B. N. Singh
  • B. N. SINGH
چکیده

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Collection of the proteus-type amoebae at the Institute of Cytology, Russian Academy of Sciences. I. History, goals and research fields

Amoebae Culture Collection at the Institute of Cytology RAS in St. Petersburg (Russia) – ACCIC (SPb) – was founded in 1960; it includes strains (clones) of large free living freshwater amoebae of Amoeba proteus-type (family Amoebidae). These protists have been extensively used as model organisms in cell biology for almost two centuries. The amoebae strains were originally received from a number...

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An electron microscope study of the nuclear membrane of Amoeba proteus by thin sectioning techniques has revealed an ultrastructure in the outer layer of the membrane that is homologous to the pores and annuli observed in the nuclear membranes of many other cell types studied by these techniques. An inner honeycombed layer apparently unique to Amoeba proteus is also described.

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The incorporation of tritiated thymidine in Amoeba proteus was reinvestigated in order to see if it could be associated with microscopically detcctable structures. Staining experiments with basic dyes, including the fluorochrome acridine orangc, revealed the presence of large numbers of 0.3 to 0.5 # particles in the cytoplasm of all cells studied. The cffcct of nuclease digestion on the dye aff...

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Cytoplasmic Dna Synthesis

The incorporation of tritiated thymidine in Amoeba proteus was reinvestigated in order to see if it could be associated with microscopically detcctable structures. Staining experiments with basic dyes, including the fluorochrome acridine orangc, revealed the presence of large numbers of 0.3 to 0.5 # particles in the cytoplasm of all cells studied. The cffcct of nuclease digestion on the dye aff...

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تاریخ انتشار 2006